| Hands-On Metabolomics and Proteomics Workshop | Dates and registration |
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This course will provide both the novice and the experienced mass spectrometry user with a comprehensive understanding of the current techniques and challenges involved in performing mass spectrometry-based proteomics and metabolomics studies using both targeted and profiling methodologies. Lecture topics: Introduction to Mass Spectrometry and Database Searching, Sample Preparation, Small Molecule Analysis, Quantitative Omics, Clinical Samples, NMR-based Metabolomics, and Advanced Technologies. Participants will also obtain extensive hands-on experience in the laboratory in the areas of sample preparation, sample collection on several mass spectrometers, and data analysis. Laboratories are divided into 3 stations where participants receive hands on experience in: Sample Preparation: Preparation of proteins and small molecule samples from urine and plasma. Instrumentation: Electrospray time-of-flight, quadrupole time-of-flight, ion trap, and triple quadrupole mass spectrometers. Curriculum: Hardware and software overview, calibration (TOFs), gradient design (LC methods), and hands-on experience on each instrument. Students analyze their own samples accumulated during the Sample Preparation laboratories using multiple instruments. Bioinformatics and Data Analysis: Students receive an overview of data analysis solutions. In addition participants use SpectrumMill and GeneSpring MS software to analyze their own data. Dates and registration |
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MetabolomicsTraining.Org
303-398-1964
1400 Jackson St., K924
National Jewish Health
Denver, CO 80206